Born in Lucerne, Regula Mühlemann studied singing with Barbara Locher at the Hochschule Luzern - Muzik. She made her first appearances on the opera stage at the Luzerner Theater, which led to a performance at the Teatro La Fenice in Venice (Despina in Cosí fan tutte). In 2012 she made her mark at the Salzburg Festival in von Winter's opera Das Labyrinth. Regula Mühlemann has received numerous awards, and was a finalist in the 2015 Cardiff Singer of the World Competition. She has performed on the most prestigious stages such as Salzburg, Berlin, Paris, Zurich, Lucerne and Geneva.
Regula Mühlemann has performed the roles of Maturina in Gazzaniga's Don Giovanni Tenorio, Barbarina in The Marriage of Figaro, Papagena in The Magic Flute, and Doralice in Scarlatti's Il Trionfo dell'onore. Recently, she played Susanna during the Salzburg Mozart Week under the direction of András Schiff. Her film credits include Hunter's Bride, based on Weber's Der Freischütz, and the adaptation of Gluck's Orfeo ed Euridice. Her debut album Mozart Arias, recorded in 2016, not only received numerous reviews but also an award from the Deutsche Schallplattenkritik (Bestenliste).
Son deuxième album Cleopatra, enregistré en 2017, a également été salué par la critique : « how captivating beautiful does the voice alloyed with gold and silver » (Sound). En 2018, elle reçoit l’OPUS Klassik « Newcomer of the Year ».